DNA
Part:BBa_K858001:Design
Designed by: Jake Sheppard, Max Jacobs, Sean Kalra Group: iGEM12_CU-Boulder (2012-09-07)
pSrgE
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
Illegal suffix found in sequence at 548 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal SpeI site found at 549
Illegal PstI site found at 563
Illegal NotI site found at 15
Illegal NotI site found at 556 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
Illegal suffix found in sequence at 549 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal XbaI site found at 24
Illegal SpeI site found at 549
Illegal PstI site found at 563 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
No mutations were made to the original sequence.
Source
This promoter came from the pJNS25 plasmid initially isolated by Ahmer et al. from Salmonella. We isolated the promoter region from the plasmid and biobricked it.