DNA

Part:BBa_K858001:Design

Designed by: Jake Sheppard, Max Jacobs, Sean Kalra   Group: iGEM12_CU-Boulder   (2012-09-07)


pSrgE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 548
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal SpeI site found at 549
    Illegal PstI site found at 563
    Illegal NotI site found at 15
    Illegal NotI site found at 556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 549
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal SpeI site found at 549
    Illegal PstI site found at 563
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No mutations were made to the original sequence.


Source

This promoter came from the pJNS25 plasmid initially isolated by Ahmer et al. from Salmonella. We isolated the promoter region from the plasmid and biobricked it.

References